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Explained: CRISPR-ERA and CRISPR/Cas9 system

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CRISPR/Cas9 system is a bacterial defence mechanism against bacteriophage infection. When a viral dna(Bacteriophage, in this case) integrates into the bacterial genome, it produces RNA which is taken up by Cas9. Continue reading “Explained: CRISPR-ERA and CRISPR/Cas9 system” »

MUSCLE: Tool for Multiple Sequence Alignment

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In my last article I discussed about the Multiple Sequence Alignment and its creation. Now in this article, I am going to explain the workflow of one of the MSA tool, i.e., MUSCLE. MUSCLE is a software which is used to create MSA of the sequences of interest.  Continue reading “MUSCLE: Tool for Multiple Sequence Alignment” »

Basic Concept of Multiple Sequence Alignment

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Multiple Sequence Alignment (MSA) is a very basic step in the phylogeny analysis of organisms. In MSA, all the sequences under study are aligned together pairwise on the basis of similar regions with in them.  The major goal of MSA pairwise alignment is to identify the alignment that maximizes the protein sequence similarity. Continue reading “Basic Concept of Multiple Sequence Alignment” »

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