The genomic complexity and unknown functions of proteins/genes in Mycobacterium tuberculosis (Mt) has triggered an in-depth study of the entire genome to explore factors responsible for influencing Mt’s behaviour at molecular level. To set the stage of infection, to establish itself in the host’s defending environment, to cause the pathogenicity by overcoming the immune system and to escape out from any assailable host attack, this TB causing pathogen has developed a well-embodied system known as two-component system (TCS) that constitutes two proteins, universally designated as sensor protein and response regulator protein.
Systems biology is not sufficient to full fill the requirement of molecular understanding of any organism at any level. It seeks to contribute multiple approaches and fields to resolve a particular issue arisen from ongoing work. In this article you will find a combinatorial approach of systems biology i.e. molecular, cellular and network biology to understand how tuberculosis is developed and how pathogen succeeds in fighting with host immune systems. A well developed mathematical model, on PhoP-PhoR two component system, is also presented and explained to demonstrate the mode of molecular regulation by pathogen. Keep Reading
In the previous section, mathematical modeling was exemplified by metabolic process and its biochemical regulation. It could also be done by signalling pathways and genetic regulatory process. At all cellular phase, one observe changing mode of a cell with effect from environmental factors. It is quite difficult to maintain cellular functions and reach to steady state. Thus, one needs to fix a range of parameters for all molecular reactions while going for mathematical modeling.