Perl programming in Bioinformatics

Perl script to find duplicate FASTA sequences using their header?

In a large file of FASTA sequences, it is nearly impossible to perform some operations manually.

This is a simple Perl script to find out duplicate sequences in a multi-fasta file using a FASTA header.

Let’s say, your multi-fasta file is ‘sequence.fasta’. 


#! /usr/bin/perl
use warnings;
use strict;

my ($infile, $header) = @ARGV;

my $duplicate;
open my $input, '<', $infile or die $!;
while (<$input>) {
    $duplicate = $1 eq $header if /^>(.*)/;
    print if $duplicate;

close $input;
Muniba is a Bioinformatician based in the South China University of Technology. She has cutting edge knowledge of bioinformatics tools, algorithms, and drug designing. When she is not reading she is found enjoying with the family. Know more about Muniba

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HOW TO CITE THIS ARTICLE Muniba Faiza (2020). Perl script to find duplicate FASTA sequences using their header?. Bioinformatics Review, 6 (06)
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