Phylogenetics
How to find a best fit model using IQ-TREE?

Previously, we have provided an installation tutorial for IQ-TREE on Ubuntu. In this article, we are going to perform model selection for a dataset using the standalone tool of IQ-TREE.
ModelFinder [1] of IQ-TREE helps to identify the best fit model for data. It supports several substitution models for DNA, protein, codon, and binary alignment.
Follow the steps given below:
- Align your sequences using any alignment software or a webserver such as Clustal, Muscle, and MAFFT.
- Save the alignment in Phylip, FASTA, MSF, NEXUS, or Clustal format.
- Now open a terminal by pressing Ctrl+Alt+T.
- Paste the following command:
$ iqtree -s aligned_seqs.fasta -m <model_name>
Here, -m refers to the model name such as TIM2+I+G, JC, TPM, WAG, and so on. You can select a model from here. - If you do not know an appropriate model for your data then use the following command to test all models:
$ iqtree -s aligned_seqs.fasta -m MFP
- If you want to resemble jModelTest/ ProtTest, then use the following command:
$ iqtree -s aligned_seqs.fasta -m TESTONLY
- You can also run the analysis on a tree as shown below:
$ iqtree -s input.phy -m <model_name>
- To re-run the analysis, use the following command:
$ iqtree -s aligned_seqs.fasta -m <model_name> -redo
References
- S. Kalyaanamoorthy, B.Q. Minh, T.K.F. Wong, A. von Haeseler, L.S. Jermiin(2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nat. Methods, 14:587-589.
Phylogenetics
Installing TREE-PUZZLE on Ubuntu

TREE-PUZZLE is a software to reconstruct phylogenetic trees using the maximum likelihood method [1,2]. It requires sequence data as input and implements a fast search algorithm and quartet puzzling. It can process large datasets easily. In this article, we will install TREE-PUZZLE on Ubuntu. (more…)
Phylogenetics
Tutorial: Constructing phylogenetic tree using MEGA7

MEGAX is a bioinformatics software/tool used for phylogenetic tree construction. In this article, we will construct a maximum likelihood (ML) tree for a number of protein sequences using MEGA7 [1]. (more…)
Phylogenetics
Update: A multi-epitope in silico vaccine candidate designed for Covid-19

Covid19 has created a great threat to human health. As you are aware, in this coronavirus outbreak, Bioinformatics Review has created a group, BiR-nCov19 Drug Development Team, to work on finding prevention to this disease. This research group consists of researchers from all over the world. (more…)
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