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AlgorithmsBioinformatics ProgrammingFasta

How to read fasta sequences from a file using PHP?

Tariq Abdullah
Last updated: October 23, 2024 7:43 pm
Tariq Abdullah
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Here is a simple function in PHP to read fasta sequences from a file.

Your multifasta input file is “input.fasta”.

function read_fastas($filename){ 
    $fh = fopen($filename, 'r'); 
    $i= 0 ; 
    $sequences = array(); 
    while($line = fgets($fh)){ 
        $i++; 
        if($i%2==1){ 
            $sequence['header'] = $line; 
        } 
        else{ 
            $sequence['sequence'] = $line; 
            array_push($sequences, $sequence); 
        } 
    } 
    return $sequences; 
}
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ByTariq Abdullah
Tariq is founder of Bioinformatics Review and Lead Developer at IQL Technologies. His areas of expertise include algorithm design, phylogenetics, MicroArray, Plant Systematics, and genome data analysis. If you have questions, reach out to him via his homepage.
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