This is a simple Perl script to extract FASTA sequences from a large fasta file depending on the matching fasta headers present in another file. Continue reading “How to extract fasta sequences from a multi-fasta file based on matching headers in a separate file?” »
This is a simple Perl script to read a multifasta file as a hash. Continue reading “How to read fasta sequences as hash using perl?” »
Roary is a pangenome genome pipeline, which calculates pangenome of a set of related prokaryotic isolates [1]. It takes annotated assemblies in the gff3 format generated by Prokka [2] and provides the pangenome. The working methodology has been explained in our previous article. In this article, we will learn how to create the pangenome of a few isolated genome sequences using Roary [1] and Prokka [2]. Continue reading “How to create a pangenome of isolated genome sequences using Roary and Prokka?” »